狼毒浸出液抗肝癌作用的实验研究

EXPERIMENTAL STUDY ON ANTI-HEPATOCARCINOMA EFFECTS OF YF-1

1目的 探讨狼毒浸出液 (YF- 1)对腹水型肝癌 (H2 2 )及淋巴道转移型肝癌 (H2 2 / F2 3 )的抗癌作用及作用机制。 2方法 用 H2 2 ,H2 2 / F2 3 细胞攻击 CFW小鼠 ,并进行 YF- 1治疗 ,观察生存时间、瘤体质量及淋巴结转移程度 ;用狼毒浸出液与 H2 2 ,H2 2 / F2 3 细胞混和培养 ,观察抑瘤率 ;用混合细胞培养液接种至小鼠腹腔 ,观察动物存活情况。 3结果 用 H2 2 ,H2 2 / F2 3攻击后 ,治疗组小鼠平均生存时间长于对照组 (t=3.16 ,3.0 4,P<0 .0 5 )。YF- 1的抑瘤率分别为 6 7.9% ,6 4.6 % .治疗组荷瘤鼠的淋巴结转移率明显低于对照组 (t=3.182 ,P<0 .0 5 )。中和实验中 ,治疗组和对照组的平均存活时间分别为 38.0 ,14.8d,两组比较差异有显著性 (t=3.16 3,P<0 .0 5 )。

Objective Using animal model of CWF mice,we studied the anti-tumor effectof YF-1(Stellera chamaejasme L) on H2 2 ,H2 2 / F2 2 hepatocarcinoma(the lympatic metastatic model) . Methods CF W inbred mice were challenged with H2 2 , H2 2 / F2 3 cells and treated with YF-1. The survival time,the weight of tumor and the lym phatic metastatic level were observed. The inhibition rate of YF-1was observed when culturing with H2 2 ,H2 2 / F2 3 cells. The survival state of the mice inoculated with the mixture of YF -1and H2 2 ,H2 2 / F2 3 was observed. Results After being challenged with H2 2 ,H2 2 / F2 3 cells,the mean survival time of CFW inbred m ice in the therapeutic group was longer than that of the control group(t=3.16 ,3.0 4;P<0 .0 5 ) .The rate of YF- 1inhibiting H2 2 ,H2 2 / F2 3 tumorcells was 6 7.9% ,6 4.6 % respectively. The lymphatic metastatic rate of the therapeutic group was lower than that of the control group(t=3.182 ,P<0 .0 5 ) .In Winn Assay,the survival time was38.0 d and14 .8d respectively (t=3.16 3,P<0 .0 5 ) . Conclusion YF -1had obvious therapeutic effect on the development and the lymphatic metastasis of hepatocarcinoma.