小肠结肠耶氏菌0:22血清型致病性研究

Detection of pathogenic ail gene in yersinia enterocolitica 0:22 serogroup

小肠结肠耶氏菌能引起人类腹泻、关节炎、阑尾炎和败血症等疾病。常见的致病性耶氏菌为0:3、0:9血清型,而0:22血清型被视为非致病型。该文用聚合酶链反应、DNA序列分析、随机扩增多态性DNA及刚果红致病性试验对其致病性进行探讨。 1,材料和方法 1.1菌株:购于中国菌种保存中心,0:22血清型3株,菌号52229;0:3血清型3株,菌号52301;

Yersinia enterocolitica can cause diarrhea dis- eases, arthritis, appendicitis, and sepsis in human be- ing. The major pathogenic serotype is 0:3, 0:9 bio- type, while 0:22 has been regarded as nonpathogenic biotype. The methods Polymerase chain reaction (PCR), DNA sequence analysis, randomly amplified polymorphic DNA (RAPD) and Congo red test were used to study the pathology of 0:22 serotype for the benefit of its provention. 1 Bacteria strains: 3 strains 0:22, No. 52229, 3 strains 0:3, No. 52301, 2 strains 0:9, No. 52302, obtained from National Institute for the control of Pharmaceutical and Biological Products, Beijing. 2 PCR: Primers was designed from ail gene seg- ment and PCR amplified as the report. 3 DNA sequence analysis: the products of PCR were pured with Clontech Co. USA PCR pure kit. The DNA segments were analyzed with PE Co. USA Genetic Analyzer. 4 Congo red test: finished according to the re- port. Result 3 strains 0:22, 3 strains 0:3 and 2 strains 0:9, were amplified by PCR, produced 359bp DNA seg- ments. Analysis of DNA sequence: 359bp PCR products of 0: 22 strain and 0: 3 were in general same. RAPD: 0: 22, 0: 3 and 0: 9 strains fingerprints were different from each other, while they had a same size major band. Congo red test: a few colon were positive with turning red. Discussion The major pathogenic serotype has been 0: 3 and 0: 9 strains, while 0: 22 has been regarded non- pathogenic. This result showed that 0: 22 strains had pathogenic ail gene as 0: 3, which were confirmed by analysis of DNA, and same colon were positive with Congo red test and were consistent with the report. RAPD fingerprints showed that there were similar in- heritance characteristics. The results showed that yersinia enteroclitica 0: 22 serotype not only has the pathogenic ail gene but also can express in some cases. It was reported that 0: 22 strain was obtained from sewage. Thus, we should paid attention to 0: 22 serotype.