神经生长因子对兔坐骨神经损伤后脊髓前角运动神经元乙酰胆碱酯酶的影响

STUDY ON THE INFLUENCE OF NGF ON ACETYLCHOLINESTERASE ACTIVITY IN MOTOR NEURONS OF ANTERIOR HORN IN RABBIT SPINAL CORD FOLLOWING SCIATIC NERVE INJURY

钳夹损伤兔右坐骨神经，于损伤处注射蛇毒ＮＧＦ４００Ｂｕ／ｋｇ／日，损伤术后１，３，７天和２，３，４，６，８周动态观察脊髓腰段伤侧第Ⅸ板层外侧群的大型运动神经元的ＡＣｈＥ活性改变。结果表明术后１，３天实验组（指损伤给药组）和对照组（指损伤对照组）ＡＣｈＥ活性均下降（Ｐ＞００５）；术后１，２，３周对照组ＡＣｈＥ活性明显下降，而实验组ＡＣｈＥ活性逐渐趋于恢复（Ｐ＜００１）；术后６周实验组ＡＣｈＥ活性恢复至正常水平（Ｐ＜００１）。本研究显示蛇毒ＮＧＦ对坐骨神经损伤后脊髓前角运动神经元ＡＣｈＥ活性恢复有促进作用。

The right sciatic nerves of rabbits in experimental and control group were crushed. The animals of the experimental group were injected with snake venom NGF (400 Bu/kg/day). 1,3,7 days and 2,3,4,6,8 weeks after nerve crush, and the changes of acetylcholinesterase (AChE) activity in motor neurons of the lumbar spinal cord were observed under LM and EM. The AChE activity in the experimental group and the control group was reduced 1,3 day after nerve injury (P>0 05). The AChE activity of the control group was decreased the during 1,2 and 3 weeks after the nerve injury (P<0 01),while the AChE activity of the experimental group was gradually recovering. The AChE activity of the experimental group retured to the normal level during 6 weeks after the nerve injury(P<0 01). The results proved that snake venom NGF could promote the recovery of the enzyme activity of AChE of the motor neuron in the anterior horn of the spinal cord following sciatic nerve crushing. Therefore, snake venom NGF may play an important role in protecting the motor neuron and accelerating the recovery of the injured motor neuron.