肺癌中增殖细胞核抗原表达、DNA含量测定及其与预后的关系

THE CORRELATION OF PCNA EXPRESSION TO DNA CONTENT AND PROGNOSIS OF LUNG CARCINOMA

本文采用ＳＰ免疫组织化学方法对６７例肺癌进行增殖细胞核抗原染色、与图像分析技术测定ＤＮＡ含量作相关性分析，探讨其在判断肺癌预后方面的意义。结果：（１）ＰＣＮＡ阳性表达强度与预后明显相关。（２）肺癌组织中ＰＣＮＡ表达强度与ＤＮＡ含量之间存在较好的相关性。（３）肺癌组织中多倍体随ＰＣＮＡ表达增强而增多，二倍体则随ＰＣＮＡ表达增强而减少。说明用简单的免疫组织化学方法检测肺癌组织中ＰＣＮＡ，以此来观察肿瘤细胞增殖活性，可作为判断肺癌患者预后的指标之一。

The expression of proliferating cell nuclear antigen (PCNA) and DNA content in 67 cases of primary lung cancer were studied with S-P immunohistochemistry of monoclonal antibody and image analysis system. The results proved: (1) The PCNA expression was closely associated with clinical TNM stages of lung carcinoma and the prognosis of patients (2) The positive grade of PCNA was associated with the DNA content in lung carcinoma (3) The histograms of DNA content show that polyploid was positively correlated with PCNA immunoreactivity while the diploid was contrary to PCNA positive grading. Therefore, the author thinks that it is very helpful to examine the PCNA by immunohistochemical method in reflect the proliferative activity of tumor cells. The PCNA is one of the useful markers to judge the prognosis of lung cancer.