C-myc基因过度表达与妊娠滋养细胞疾病预后关系的探讨

The Relationship between Overexpression of C-myc and Prognosis of Gestational Trophoblastic Disease

采用核酸分子杂交技术，以地高辛dig－11－dUTP标记的C－myc基因探针与54例妊娠滋养细胞疾病（GTD）石蜡包埋组织（其中葡萄胎、侵蚀性葡萄胎、绒癌各18例）进行原位杂交，定性、半定量地测定了C－myc基因在GTD组织中的表达，结果提示：C－myc基因随着GTD病变的发展，其表达呈逐渐增高的趋势：C－myc基因与恶性滋养细胞肿瘤的临床分期及预后有关，即临床分期越晚，C－myc阳性表达率越高；年龄≥40岁、病程＞1年、前次妊娠为非葡萄胎、恶性程度高及有转移组的患者，其C－myc阳性表达率增高。

The expression of C-myc gene in paraffin embedded tissues of 54 cases of GTD was qualita-tively, half-quantitatively measured by in situ hybridization using a dig-11-dUTP-labelled C-myc gene probe.The result showed that ① the expression of C-myc gene increased with the malignancy of GTD;②it was re-lated to clinical stage and prognostic factors of GTT, that is. the more advanced was the clinical stage, the higher positive expression of C-myc; ③C-myc gene hyperexpressed in those over 40 years of age, lasting more than one year, with non-hydatidiform mole in the last gestation, high malignancy and metastasis.