电磁场促进成骨细胞体外骨发生钙离子调节机制

The Effects of Magnetic Fields Stimulation on Osteogenesis and the Mechanism Mediated by Ca 2+

通过观察电磁场刺激对体外培养成骨细胞骨生成过程的作用，为临床骨伤病的治疗提供理论依据和技术方法．用体外培养兔胫骨膜成骨细胞，实验组置于２Ｈｚ，１Ａ·ｍ－１的梯形脉冲电磁场中，对照组无电磁场刺激，观察成骨细胞的增殖速度和分泌功能的差异，测量细胞内游离钙离子含量水平．结果：成骨细胞在ＤＭＥＭ＋１０％小牛血清培养基中生长良好，体外培养８ｄ可融合成一层，然后重叠生长，形成岛状骨结节，结节中部成骨细胞随着钙盐沉积坏死，少量成骨细胞向骨细胞转化，实验组成骨细胞分化生长速度明显加快．结论：体外培养成骨细胞在适宜的环境中，以膜内成骨的方式生成骨结节，这一过程可被电磁场刺激加快速度．电磁场刺激促进成骨细胞生长分化的机制与调节细胞内游离钙离子代谢有关．

The experimental model of in vitro culture and magnetic field stimulation of isolated rabbit osteoblastlike cells was used to study the effects of magnetic fields environments on osteogenesis and bone metabolism.Ca 2+ concentration in the osteoblastlike cells were measured with an Adherent Cell Analysis and Sorting Interactive Laser Cytometer (ACAS-570).The results showed that 2 Hz,1A·m -1 magnetic field stimulation is effective in opering the Ca 2+ passages in the osteoblastlike cells,increasing the intracellular concentration of free calcium ions and fasting the in vitro proligeration of the cells.