G蛋白反义寡核苷酸对血管平滑肌细胞增殖的影响

THE EFFECTS OF G PROTEIN ANTISENSE OLIGODEOXYNUCLEOTIDES ON PROLIFERATION OF VASCULAR SMOOTH MUSCLE CELLS

使用硫代修饰的Ｇ蛋白（Ｇαｑ／１１亚基和ｒａｓ蛋白）反义寡聚脱氧核苷酸（Ｇαｑ／１１亚基和ｒａｓ蛋白ＡＳ－ＯＤＮｓ）作用于含２０％胎牛血清ＤＭＥ培养液培养基的血管平滑肌细胞（ｖＳＭＣ）。通过细胞计数和免疫细胞化学方法，观察了Ｇαｑ／１１亚基和ｒａｓ蛋白ＡＳ－ＯＤＮｓ对ｖＳＭＣ增殖和ＰＣＮＡ表达的影响。结果表明，Ｇαｑ／１１亚基和ｒａｓ蛋白ＡＳ－ＯＤＮｓ联合应用能明显抑制ｖＳＭＣ增殖；免疫细胞化学分析亦显示ｖＳＭＣ中ＰＣＮＡ蛋白表达显著减少。而相同浓度的正义ＯＤＮｓ只有微小抑制作用。结果提示，Ｇαｑ／１１亚基和ｒａｓ蛋白ＡＳ－ＯＤＮｓ有可能应用于防治临床血管活性物质依赖性细胞增生性疾病的研究。

Antisense phosphorothioate oligodeoxynucleotides(AS ODNs) were added to vSMC cultured in 20% fetal calf serum DME media.Using cell count and immunocytochemistry methods,the cell growth and expression of proliferating cell nuclear antigen (PCNA) of vSMC were studied.Results showed that the joint administration of Gαq/11 subunits and small molecular G protein(ras rotein)AS ODNs could significantly inhibit vSMC proliferation.Immunocytochemistry analysis show that expression of PCNA protein in vSMCs also decreased evidently.When Gαq/11 subunits and ras protein sense ODNs were used instead of AS ODNs,only a little effect was demonstrated.In conclusion,Gαq/11 subunits and ras protein AS ONDs may be used for clinical research of prevention and treatment of vasoactive factors dependent proliferating diseases.