转化生长因子-β_1在肝细胞癌的表达

THE EXPRESSION OF TRANSFORMING GROWTH FACTOR β 1 IN HEPATOCELLULAR CARCINOMA

用ＬＳＡＢ法对１２例肝细胞癌病人手术切除标本进行转化生长因子－β１（ＴＧＦ－β１）免疫染色，经计算机图象分析系统分析，发现肝癌组织的ＴＧＦ－β１表达明显低于癌旁肝组织，阳性染色面积（相对数），肝癌与癌旁肝组织分别为（９．９６±１０．７）％和（５６．６±２６．６２）％（Ｐ＜０．０１）。此外，外周血ＴＧＦ－β１水平测定（ＳＥＬＩＳＡ法）发现，健康志愿者组和肝硬化病人组外周血ＴＧＦ－β１水平明显高于肝癌病人（未治疗组），３组分别为（１８２．９２±９０．４５）μｇ／Ｌ，（１１９．５３±８７．７３）μｇ／Ｌ和（６２．３７±５１．５２）μｇ／Ｌ（Ｐ＜０．０１和Ｐ＜０．０５）；本研究提示肝癌细胞ＴＧＦ－β１表达降低可能与其细胞的转化和生长失控有关，也是其生物学特征之一。

Transforming growth factor β 1 (TGF β 1) is an important mediator of control of liver cell proliferation and differentiation. The aim of the current study was to compare TGF β 1 expression in neoplastic human livers and normal liver beside neoplasm. Twelve resections for hepatocellular carcinoma were included in this study. TGF β 1 protein were detected on serial tissue sections using immunohistochemistry. The results were analysed by computerized image analysis system. In liver beside neoplasm, TGF β 1 was significantly expressed. The amount of positive area was respectively (9.96±10.7)% and (56.6±26.62)% ( P <0.01). Plasma TGF β 1 levels in patients with HCC ( n =15) were compared with those in patients with viral hepatitis cirrhosis ( n =14) and in normal subjects ( n=10 ) using an enzyme linked immunosorbent assay system. Normal subjects and the patients with cirrhosis had significantly higher plasma TGF β 1 levels [(182.92±90 45)μg/L and (119.53±87.73)μg/L] than those in patients with HCC [(62.37±51.52)μg/L] ( P <0.01). This study suggest that the decrease expression of TGF β 1 by tumoral hepatocytes might be related to its transformation and unlimited proliferation.