摘要
以党参为实验材料，在附加1mg／L2，4-D3％或7％蔗糖的MS培养基上，成功获得体细胞胚胎发生同步化较高的实验体系。用ELISA方法测定了胚性细胞形成球形胚的过程中，内源ABA和CTK的含量变化。结果表明，在这一过程中内源ABA含量持续增加；内源CTK的组分和含量均发生很大变化，表现在组分iPAs在球形胚形成前急剧增加，球形胚形成期急剧下降，组分ZRs在球形胚形成前上升较缓慢，球形胚形成期急剧上升。
Synchronization of Codonoposis pilosula somatic embryogenesis was successfully gained,the callus cultured in MS medium containing 1 mg/L 2,4-D and 3% or 7% sucrose. Endogenous abscisic acid(ABA) and cytokinins(CTK) were determined by ELISA during early stage from embryonic cell to globular embryo. The results showed that ABA content increased continously and CTK quantity and quality both changed remarkably,iPAs content increased during embryonic cell masses phase and decreased during
出处
《西北植物学报》
CAS
CSCD
1997年第6期146-152,206,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
博士点基金
