胰腺癌K-ras基因12密码子突变率的研究

A STUDY ON THE MUTATION RATE OF K-ras CODON 12 IN PANCREATIC ADENOCARCINOMA

目的阐明中国胰腺癌患者Ｋ－ｒａｓ基因１２密码子突变频率，为临床应用打下基础。方法两轮ＰＣＲ－ＲＦＬＰ技术对３２例石蜡包埋胰腺癌标本和１５例阴性对照作基因分析。结果３２例胰腺癌标本中３０例检测到Ｋ－ｒａｓ基因１２密码子突变，阳性率为９３．８％，而１５例阴性对照无１例检测到突变。结论中国胰腺癌患者Ｋ－ｒａｓ基因１２密码子突变率高达９０％左右，该突变的有无可作为在基因水平上鉴别诊断胰腺癌的一个可靠标志。

Objective To investigate the mutation rate of K-ras codon 12 in Chinese patients with pancreatic adenocarcinoma and assess its value for clinical application.Method 32 paraffin-embedded specimens of pancreatic adenocarcinoma and 15 negative controls were analysed at gene level by using double PCR-RFLP.Result K-ras codon 12 mutation was present in 30 out of 32 cases of pancreatic adenocarcinoma,the positive rate was 93.8%,whereas no mutation was detected in the 15 negative controls.Conclution The mutation rate of K-ras codon 12 is up to about 90% in pancreatic adenocarcinomas in China and this mutation may serve as a reliable marker for distinguishing and diagnosing pancreatic adenocarcinoma at gene level.