星形细胞瘤中肿瘤抑制基因及生物学标记表达的研究

Immunohistochemistry studies of the expression of the tumor suppressor and the biologic indicator in astrocv tomas

目的:检测30例不同病理级别星形细胞瘤中p53基因的表达状况及肿瘤其它生物学特性.方法:采用常规HE染色及免疫组织化学技术检测30例标本中p53、PCNA、GFAP、VIM、S-100的表达.结果:P53免疫组化染色阳性率随病理级别增高而增高,Ⅳ级阳性率 83.3%,Ⅰ、Ⅱ级15.4%,且不同级别染色程度也有差异;PCNA均呈阳性,Ⅳ级病例染色阳性强度大于Ⅰ级.GFAP、S-100随病理级别升高而阳性率降低,VIM却随级别升高而升高.结论:检测P53基因突变产物,可判断星形细胞瘤恶性程度与预后,P53基因在星形细胞瘤发展、演化中有重要作用.PCNA可反映星形细胞瘤增殖活性及恶性程度,GFAP、VIM、S-100的测定也可了解其分化程度,三者结合,可增加判断的准确性.

To detect the existing situation of p53 gene expression and other biological characteristics from 30 patients with astrocytomas. Method: All the cases of astrocytoma were detected by immunohistochemistry, using corresponding monocolonal antibodies with strepta-vidin peroxdase conjugataed method ( SP method). Result: The positive ratio of nuclear p53 staining was higher in astrocytoma of grade IV than in that of grade Ⅰ ~ Ⅱ (IV 83.3%, Ⅰ- Ⅱ 15.4%). The density of staining in grade Ⅳ was also deeper than that in grade Ⅰ - Ⅱ . However, the p53 staining was unrelated to patient ' s age and sex. All the samples were positⅣe in PCNA immunostaining. Tumors of grade Ⅳ had much more dense PCNA staining (3 + in 54.5% ),than that of grade Ⅰ - Ⅱ tumors ( + in 57. 1 % ). The ratio of GFAP and S-100 positⅣe staining is higher in grade Ⅰ ~ Ⅱ than that of grade Ⅳ , while VIM is higher in grade Ⅳ than that of grade Ⅰ - Ⅱ( I -Ⅱ 35 % , Ⅳ 45 %). Conclusion: The mutation of p53 gene is related to the degree of tumor malignancy and patient's prognosis. PCNA might be a good indicator for the proliferatⅣe activty and the grade of malignancy of astrocytoma. The detections of GFAP,VIM and S-100 also aid to assess the cellular differentiation of tumors, they can play an important role in understanding the characteristics of astrocytoma more accurately if used in combination.