摘要
目的观察ＦＫ５０６对大鼠肾转化生长因子－β（ＴＧＦ－β）和纤维连结蛋白（Ｆｎ）基因表达的影响。方法对ＦＫ５０６组８只大鼠行部分肾切除术以造成与人肾移植相似的模型。以Ｎｏｒｔｈｅｒｎ杂交方法，采用鼠ＴＧＦ－β和Ｆｎ基因探针检测大鼠肾ＴＧＦ－β和Ｆｎ基因的表达。结果大鼠肾ＴＧＦ－β和Ｆｎ基因的表达在ＦＫ５０６组明显高于对照组。以光密度分析仪将杂交斑点密度转换为吸收度，发现ＦＫ５０６组ＴＧＦ－β和Ｆｎ基因的表达水平分别是对照组的３．５倍和２倍。结论ＦＫ５０６可以增强大鼠肾ＴＧＦ－β和Ｆｎ基因的表达，促纤维合成细胞因子和糖蛋白在ＦＫ５０６诱导肾毒性中的作用可能与此有关。
Objective To observe the effect of FK506 on expression of transforming growth factor-β (TGF-β) and fibronectin(Fn) genes in rat kidney.Methods Nephrectomy was performed on 8 rats.With rat TGF-β and Fn probe,the expression levels of TGF-β gene and Fn gene in 8 rat kidney were analyzed by Northern blot.Results Compared with control group,the expression levels of TGF-β gene and Fn gene were relatively hygh in FK506 group kidney.By densitometric analysis,350% change was found over control group kidney in TGF-β gene expression and 200% in Fn gene expression.Conclusion The present study demonstrates that FK506 enhances TGF-β and Fn gene expression in rat kidney,which may be associated with the roles of fibrosis-promoting cytokine(TGF-β)and glycoprotein (Fn) in FK506-induced nephrotoxicity in rats.
出处
《徐州医学院学报》
CAS
1997年第5期55-58,共4页
Acta Academiae Medicinae Xuzhou
