壮督方对强直性脊柱炎患者T辅助细胞亚群的影响

Impact of Zhuangdu Recipe on Th Cell Subsets in Ankylosing Spondylitis

目的 :探讨T淋巴细胞亚群与强直性脊柱炎 (AS)发病的关系以及壮督方对AS患者T辅助细胞亚群的调节作用。方法 :采用酶联免疫斑点法 (Elispot)对AS患者和健康志愿者外周血中T辅助淋巴细胞进行分析。结果 :AS患者外周血中以Th1型细胞为主 ,AS患者组外周血中Th1细胞比正常组明显升高 ,AS患者外周血中Th1、Th2水平与炎症活动指标ESR、CRP具有一定的相关性。经用壮督方治疗 6个月后AS患者外周血中Th1细胞数下降而Th2细胞数上升。结论 :经壮督方治疗后 ,AS患者外周血中Th1细胞有下降趋势、Th2细胞有上升趋势 。

Objective: To discuss the relationship between the Th cell subsets and AS and to explore the regulating impact of Zhuangdu Recipe on Th cell subsets in AS by measuring the Th1 and Th2 cell level in peripheral blood among ankylosing spodylitis (AS) patients and normal person. Methods: Elispot method was employed for the analysis of Th cell subsets in the peripheral blood of AS patients before and after the treatment with Zhuangdu Recipe and in that of the healthy volunteers. Results: Th1 cell subset in the peripheral blood of the AS patients was obviously higher than that of the normal control group. There was correlativity between the level of Th1 and Th2 levels and ESR/CRP the indices of inflammatory activities. After being treated with Zhuangdu Recipe, the number of Th1 cell decreased and the number of Th2 cell increased. Conclusion: Zhuangdu Recipe can improve the imbalance of Th cell subsets in the peripheral blood of the AS patients.