用PCR-RFLP方法分析流感病毒H_3N_2亚型毒株

Analysis n HA_1 fragment of influenza H_3N_2 with PCR-RFLP

采用PCR－RFLP方法，将1996年流感病毒H3N2亚型分成阿拉斯加变异株系和武汉变异株系；分析发现，全球流感病毒H3N2亚型以武汉变异株系为优势毒株。来自中国的28份流感病毒H3N2亚型均为武汉变异株系；美国主要流行武汉变异株系（41／50），其次为阿拉斯加变异株系；世界其它地区以武汉变异株系为主。作者认为，PCR－RFLP方法作为一种分子流行病学筛选实验，在流感变异研究中具有重要作用。

The strains of Influenza H3N2 isolated in 1996 were classified into two lineages including Alaska-Lineage and Wuhan-Lineage with the method of PCR-RFLP. It was found that the Wuhan-Lineage was the prevalcnt strains in the world in 1996. The 28 isolates from China were all identified into the Wuhan-Lineage, whilc the Wuhan-Lineage was the prevalent strains in the United States (41/50), as Alaska-Lineage was the circulating strains. Wuhan-Lineage was the prevlent strains in the rest of the world too. PCR-RFLP was considered as a screening method of influenza variation in molecular-epidemiology.