人乳头状瘤病毒与肛管癌关系——PCR、斑点杂交、免疫组化、原位杂交分析

RELATION BETWEEN HUMAN PAPILLOMAVIRUS INFECTION AND ANAL CARCINOMAS——ANALYSES BY THE POLYMERASE CHAIN REACTION, DOT BLOT HYBRIDIZATION, IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION

用聚合酶链反应、斑点杂交、原位杂交、免疫组化技术检测７２例肛管癌标本，ＰＣＲ扩增１９例鳞癌１例阳性（５％），斑点杂交证实为ＨＰＶ１６型，免疫组化发现ＨＰＶ抗原不仅在癌巢中，还在癌周上皮浅层和正常腺体中分布，但原位杂交检测阴性。其余标本检测均呈阴性。研究表明本组肛管鳞癌与ＨＰＶ感染关系不密切，但提示ＨＰＶ可以感染腺上皮。

In this study the polymerase chain reaction (PCR), dot blot hybridization (DBH), in situ hybridization (ISH) and immunohistochemistry (IMHC) techniques were applied to detect HPV DNA and antigen in 75 specimens of anal carcinomas. The results showed that in 19 squamous cell carcinomas (SCC), HPV,DNA was detected only in one case (5%, 1/19) by PCR, and was further identified as HPV 16 by DBH . HPV antigen was also found in this case. Positive staining was present not only in foci of the invasive tumour, but also in nonneoplastic superficial epithelium and nomal glands adjacent to the tumor. However, this study failed to detect HPV in other anal cancers and rectal adenocarcinomas. We found that the HPV infection was not closely associated with the anal cancers in this group, however it existed in the anal glandular epithelium.