卵巢粘液性肿瘤P53蛋白表达的免疫组织化学定量研究

IMMUNOHISTOCHEMICAL QUANTITATIVE STUDY ON THE EXPRESSION OF P53 PROTEIN IN OVARIAN MUCINOUS TUMORS

采用ＬＳＡＢ法对１０例卵巢粘液性囊腺瘤、２１例交界性粘液性乳头状囊腺瘤、３０例粘液性乳头状囊腺癌，进行了Ｐ５３蛋白的免疫组化法检测。结果：Ｐ５３蛋白的表达率在囊腺瘤为阴性，交界性囊腺瘤为１９％，囊腺癌为５３．３％。囊腺癌的表达率显著高于囊腺瘤（Ｐ＜０．００１）、交界性囊腺瘤（Ｐ＜０．０２５）。图像定量分析结果，囊腺癌的阳性细胞平均积分光密度值（３．６３±２．２０）显著高于交界瘤的值（１．６８±３．４９），（Ｐ＜０．００１）。Ｐ５３蛋白的表达与囊腺癌的不同组织学分级和临床分期之间无相关性（Ｐ＞０．０５）。上述结果提示，Ｐ５３基因突变参与了卵巢粘液性肿瘤由良性向恶性转化的过程。

In order to study the significance of the expression of P53 protein in the ovarian mucinous tumours, 10 ovarian mucinous cystadenomas (MC), 21 borderline mucinous cystadenomas (BMC), and 30 mucinous cystadenocarcinomas (MCC) were examined by using immunohistochemical LSAB technique. The result showed that the expression rate of p53 protein in cystadenoma was negative, in BMC 19% and in MCC 533%. The expression rate of MCC was significantly higher than that of benign MC (P<0001) and BMC (P<0025). Also the quantitative analysis of p53 protein expression was studied by CMIAS computer image analysis system in 20 cases of BMC and MCC. It was found that the average integral optical density (IOD) of the positive cells of MCC was significantly higher than that of BMC. The expression of p53 protein was not related to the histological grading and clinical phase of the ovarian mucinous tumors. The above findings suggested that the expression of p53 protein might be the mark of the transformation of ovarian mucinous tumours from the benign to malignant state.