蛋白激酶底物p36在肝硬变、肝细胞肝癌中的表达及与HBV、HCV感染的关系

EXPRESSION OF PROTEIN KINASE SUBSTRATE P36 IN LIVER CIRRHOSIS AND HEPATOCELLULAR CARCINOMA AND ITS RELATIONSHIP WITH HBV AND HCV INFECTIONS

本文应用鼠抗蛋白激酶底物ｐ３６单克隆抗体，采用免疫组织化学法对ｐ３６在５４例肝硬变，７９例肝细胞肝癌中的表达分布进行了研究，同时结合ＨＢＶ、ＨＣＶ感染情况分析其相互关系，结果显示：ｐ３６在肝硬变及肝细胞肝癌中定位于肝细胞或癌细胞胞浆内，在胞浆内弥漫分布，阳性细胞呈灶状或弥漫分布，部分病例癌周肝细胞信号较癌组织为强，ｐ３６在肝硬变、肝细胞癌中的阳性率分别为８８．８％（４８／５４）及８２．３（６５／７９），ＨＢｘＡｇ在两种组织的阳性率分别为７０．４％及７６％，ＨＣＶ核心抗原在两种组织的阳性率分别为８０％及７８．５％；三者同时阳性分别为５５．５％及５８．２％；ｐ３６、ＨＢｘＡｇ同时阳性分别为６８．５％及６４．５％；ｐ３６、核心抗原同时阳性分别为７４．１％及７０．８％，我们的结果提示，肝硬变、肝细胞肝癌组织中ｐ３６存在高表达，其高表达可能与ＨＢＶ。

The expression and distribution of protein kinase substrate p36 were studied in 54 cases of liver cirrhosis and 79 cases of hepatocellular cacinoma tissues with monoclonal antibody against p36 by immunohistochemistry method. Combining with the state of HBV and HCV infection, and their corelationship was analysed. The results showed: The p36 was localized in the cytoplasm of hepatocyte and cancerous cells. Positive substance distributed diffusely in cytoplasm. The positive cells distributed in locals or diffusion. In some cases the positive signal in pericancerous liver tissues was stronger than that of cancerous tissues. The positive rates of p36, HBxAg and HCV core antigen in liver cirrhosis and hepatocellular carcinoma were 88 8%(48/54) and 82 3%(65/79),70 4%(38/54) and 76%(60/79),80%(43/54) and 78 5%(62/79) respectively. HCV core antigen, p36 and HBxAg positive rates at the same time were 55 5%(30/54) and 58 2%(46/79) in liver cirrhosis and hepatocellular caricnoma. p36 and HBxAg double positive rates were 74 1%(40/54) and 70 8%(56/79). Our results suggest that there is high expression of p36 in liver cirrhosis and hepatocellular carcinoma and the high expression of p36 may be closely related to HBV, HCV infections.