小鼠胸腺哺育细胞内淋巴细胞碱性磷酸酶电镜观察

ELECTRON MICROSCOPIC OBSERVATION OF ALKALINE PHOSPHATASE OF LYMPHOCYTE WITHIN THYMIC NURSE CELL (TNC) IN MICE

碱性磷酸酶（ａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）是研究Ｔ淋巴细胞分裂分化的标记之一。胸腺内淋巴母细胞具有膜性ＡＬＰ。本文采用ＡＬＰ超微结构细胞化学方法，探讨小鼠胸腺哺育细胞（Ｔｈｙｍｉｃｎｕｒｓｅｃｅｌ，ＴＮＣ）内淋巴细胞（ＴＮＣ－Ｌ）ＡＬＰ的活性分布。观察结果，２０％的ＴＮＣ内有少数淋巴细胞呈阳性反应，而ＴＮＣ外游离的淋巴细胞均为ＡＬＰ阴性反应。结果表明，部分ＴＮＣ内含有少量淋巴母细胞亚群。

Alkaline phosphatase(ALP) is one of the markers used to study the division and differentiation of T Lymphocyte. The lymphoblasts in thymus possess membrane ALPase activity. In our studies the distribution of ALPase activity of lymphocytes within thymic nurse cell(TNC-L) was investigated by means of an ultrastructural cytochemical method. The observation revealed that within 20% TNC-L a small number of lymphocytes showed ALPase-positive activity, however, the free lymphocytes outside TNC appeared as the negative reaction. The result indicates that there are a few subpopulations of lymphoblast in parts of TNC and TNC is one of the microenvironments for the Tlymphocyte division and differentiation.