摘要
构建了由PGK启动子驱动HSV-tk基因的重组质粒pKGTK,反转录病毒载体pLNTK,并成功地转移至小鼠神经母细胞瘤NBA<sub>2</sub>细胞内。体外实验证实NBAKGTK和NBALNTK对阿昔洛韦(acyclovir,ACV)的杀伤敏感性分别为NBA<sub>2</sub>约100倍和500倍。应用<sup>3</sup>HTdR掺入法检测DNA的合成能力,证实ACV能够明显抑制NBAKGTK和NBALNTK的DNA合成。共培养实验证实存在旁观者杀伤效应。
The paper reports the construcation of expression vector pKGTK and retroviral vector pLNTK carrying HSV-tk gene by PGK promoter and successful transfer into mice neuroblastoma cell NBAj. The in vitro study confirmed that acyclovir (ACV) sensitive level of the genetransferred neuroblastoma cell (NBAKGTK, NBALNTK) was 100 and 500 times that of NBA2 .respectively. 3H-TdR incorporation confirmed that the DNA replication in NBAKGTK and NBALNTK was considerably suppressed when treated with ACV. The increment of ACV sensitive level of parent cells when cocultured with gene modified cell (1 ! Dsuggested the existence of bystander effects.
出处
《沈阳医学院学报》
1997年第1期1-4,11,共5页
Journal of Shenyang Medical College