沈阳地区丙型肝炎病毒感染的基因分型与临床意义

Genotypes of HCV Infection in Shenyang Area and Their Clinic Significance

应用引物特异PCR法对沈阳地区95例各型丙型肝炎患者血清抗HCV及HCV RNA均阳性的标本进行丙型肝炎病毒HCV基因分型,旨在了解本地区HCV基因型分布情况与临床病情的相关性。结果表明,HCV—Ⅱ型感染占58.9％(56/95),Ⅲ型占28.4％(27/95),Ⅱ/Ⅲ混合型感染占11.6％(11/95),未分型占1.1％(1/95)。急性肝炎、慢性肝炎轻度、中度、重度以及肝炎后肝硬化中HCV—Ⅱ型感染分别占63.6％,36.2％,72.7％,87.5％和92.3％;而HCV—Ⅲ型感染分别占27.3％,46.8％,9.1％,0％和7.7％。说明沈阳地区HCV感染以Ⅱ型为主,其次为Ⅱ型及Ⅱ/Ⅲ混合型,HCV基因型与肝病严重程度呈密切相关。

To obtain the relationship between HCV genotype distribution and clinic infection in Shenyang area, the genotypes of 95 serai samples from the patients whose anti - HCV antibodies and HCV RNA were both positive were typed by the primer-specific PCR technic. The results showed that 58. 8%(56/95) of genotypes were HCV- I , 28. 4%(27/95) HCV-I , 11. 6%(11/95) HCV- I /I and 1. l%(l/95) not types. In the samples from acute hepatitis, chronic mild, moderate and heavy type hepatitis and posthepatitis cirrhosis, HCV- I infection rates were 63. 6%, 36. 2%, 72. 7%, 87. 5% and 92. 3% respectively, and HCV- I were 27. 3%, 46. 8%, 9. 1%, 0% and 7. 7% respectively, All above indicated that HCV- I was the major infection genotype and others were HCV- I and HCV-I /1 . There was a closed relationship between HCV genotype and the serious extent of clinic infection. *