摘要
以正常肝和再生肝为对比材料,利用mRNA差别显示技术,研究肝再生过程中基因的差别表达,旨在从基因选择性表达水平上探讨肝再生调控机理。得到4种未知肝再生相关cDNA,完成其克隆与序列分析,其一在再生肝中的表达低于正常肝,余者则高于正常肝。这些序列均已在EMBL(Europe Molecular Biology Laboratory)数据库中登录,登录号分别为X95721、X95722、X95723、X97973。
Regeneration of liver after hepatectomy is a complex process. It has been thought that there might be some growth factors and genes which play important roles in this process. In order to understand the regulatory mechanism of liver regeneration at molecular level, the changes in gene expression of regenerating liver have been demonstrated by mRNA differential display. As a result, four candidate cDNA fragments were obtained, including one down-regulated and three up-regulated. DNA sequencing demonstrated that all of them were novel. These sequences have been assigned the database accession numbers as below: X95721 ,X95722,X95723,X97973.
出处
《生物技术通讯》
CAS
1997年第1期10-13,共4页
Letters in Biotechnology
基金
国家863计划资助
关键词
肝再生调控
MRNA差别显示
基因克隆
核苷酸序列分析
regulation of liver regeneration
mRNA differential display
gene cloning
sequencing of DNA