老年病毒性肝炎和肝细胞癌患者外周血可溶性白细胞介素2受体的检测及意义

SIGNIFICANCE OF SOLUBLE INTERLEUKIN - 2 RECEPTORS IN PERIPHERAL BLOOD OF ELDERLY PATIENTS WITH VIRAL HEPATITIS AND HEPATOCELLULAR CARCINOMA

应用单克隆抗体夹心酶联免疫吸附试验(ELISA)对75例老年病毒性肝炎和肝细胞癌患者血清的可溶性白细胞介素2受体(sIL-2R)进行检测,并与健康老年人作对照比较。结果显示,急性肝炎黄疸期、慢性肝炎(中度)、重型肝炎组血清sIL-2R分别为129.36±37.15/ml、174.44±91.90u/ml、245.00±77.67u/ml,明显高于正常对照组的72.67±43.46/ml(P<0.01)。而慢性肝炎(轻度)、急性肝炎恢复期、肝癌组分别为82.67±30.86u/ml、73.61±20.28u/ml、70.12±30.36u/ml,与正常对照组比较无显著差异(P>0.05)。sIL-2R水平与感染的肝炎病毒类型无关(P>0.05)。老年肝癌组经免疫增强型治疗后血清sIL-2R水平升高至207.14±88.45u/ml(P<0.05)。血清sIL-2R含量的消长与疾病严重程度似呈正相关,提示临床检测sIL-2R水平对判定病情变化和预后及评估机体免疫状态有一定的意义。老年人免疫力低下可能是肝癌的重要致病因素之一。

The levels of serum soluble interleukin-2 receptors (sll-2R) were measured in 75 patients ( >60 years old) with viral hepatitis and hepatocellular carinoma and in 30 cases of normal control by enzyme linked immunosorbent assay (ELISA).The serum sIL-2R levels in patients with acute viral hepatitis (icteric stage), chronic active hepatitis and severe hepatitis were 129.36 + 37.15 u/ml, 174.44 + 91.90 u/ml and 245.00 +77.67 u/ml, which were significantly higher than in normal control group (72.67 +43.46 u/ml)(p<0.01).But in those with chronic persistent hepatitis.acute viral hepatitis (convalescent stage) and hepatocellular carcinoma were 82.67 + 30.86 u/ml, 73.61 +20.28 u/ml, 70.12+ 30.36 u/ml, not significantly different as compared with normal control group (p> 0.05).No significant difference in sIL-2R levels was found among patients with human hepatitis A, B, C, and E(p> 0.05).The serum sIL-2R levels in patients with hepatocellular carcinoma after administration of Zadxin were increased obviously (207.14 + 88.45 u/ml) (p<0.05).The patients with severe hepatitis had the highest sIL-2R levels, while those with acute viral hepatitis had the lowest levels among the different hepatitis groups.which seems to suggest that the serum sIL-2R levels was associated with the degree of hepatic injuries.