国产胸腺肽对健康人PBMC膜白细胞介素2受体和HLA-DR抗原表达的影响

THE EFFECT OF THYMOPEPTIDES ON THE mIL - 2R AND HLA - DR EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS FROM HEALTHY DONORS

用生物素-链霉亲和素系统免疫细胞化学和流式细胞仪检测mIL-2R和HLA作为考核胸腺肽体外活性的指标,结果表明,25～100μg/ml胸腺肽在体外明显提高mIL-2R和HLA-DR表达率,提示胸腺肽具有激活淋巴细胞的功能,为胸腺肽治疗慢性肝炎的机理提供了理论基础。

By means of BSA immunocytochemistry and flow cytometer, we have studied the effects of thy-mopeptide on mIL-2R and HLA-DR expression of in vitro cultivated PBMC from healthy donors.The results showed that the percentages of mIL-2R and HLA-DR+ cells were remarkably increased in PBMC stimulated by 25 ~ 100 ug/ml of thymopeptide culture.It is suggested that the thy-mopeptides have potential PBMC activation activity in healthy donors.The expression of mIL-2R and HLA-DR in PBMC can be regarded as indicators of assessing the thymopeptide therapeutic effect.