酒精性肝硬变患者血清干扰素和可溶性干扰素受体的检测及意义

DETERMINATION OF SERUM INTERFERON AND SOLUBLE INTERFERON RECEPTOR IN PATIENTS WITH ALCOHOLIC CIRRHOSIS

酒精性肝硬变(AC)常伴有体液免疫和细胞免疫系统功能改变,干扰素(IFN)和可溶性干扰素受体(sIFNR)是免疫细胞的重要调理素。为了进一步弄清IFN和sIFNR与AC之间的关系,我们用ELISA法检测了32例AC患者和10例正常人血清IFN和sIFNR水平。结果发现AC组α-IFN,γ-IFN和sIFNR的血清浓度明显高于正常人(P值分别<0.01,<0.05和<0.05),但其变化与疾病的严重程度无相关性。这些结果提示AC患者存在血清α-IFN、γ-IFN和sIFNR的改变。

Alcoholic cirrhosis (AC)is accompaned by alteration of humoral and cellular immune functions.In-terferon (IFN) and soluble interferon receptor (sIFNR) are important regulators of immune system.The existence of a cellular immune disturbance in cirrhosis led us to determine serum concentrations of these lymphokines in an attempt to shed light on their role in immune alterations associated with AC.Their serum levels in a gruop of 32 patients with AC classified according to Child-Pugh's class of liver disease severity and of 10 healthy controls were determined by enzyme-linked immunosorbent assay (ELISA).Serum levels of a-IFN, y-IFN and sIFNR were markedly increased in patients with AC as campared to in healthy controls (p<0.01,p<0.05,p<0.05, respectively), While there was no significant difference between the serum levels and the different class of hepatic damage.These findings suggest that a profound alteration of serum a-IFN,7-IFN and sIFNR levels may exist in human alcoholic cirrhosis.