摘要
Using flow cytometry analysis with human type Ⅰinterferons (IFN) (IFN α and β) and IFN α/β receptor antibody (ligand binding and antibody binding), we compared human IFN α/β receptor expressions on human H9 (T cells), Raji (B cells) and U937 (monocyte cells). The results indicated that H9, Raji and U937 cells all expressed IFN α/β receptor on their cell surfaces and the Raji cells showed the highest level of expression. The flow cytometry analysis using the antiserum to human IFN α/β receptor more clearly showed a strong IFN α/β receptor expression on Raji cells and a weak expression on H9 and U937 cells. These results indicate that B cells (Raji) expressed more receptor molecules on their cell surface than on T cells (H9) and on monocytes (U937), and the number of receptor molecules expressed on the cells is enough to be measured with flow cytometry.
Using flow cytometry analysis with human type Ⅰinterferons (IFN) (IFN α and β) and IFN α/β receptor antibody (ligand binding and antibody binding), we compared human IFN α/β receptor expressions on human H9 (T cells), Raji (B cells) and U937 (monocyte cells). The results indicated that H9, Raji and U937 cells all expressed IFN α/β receptor on their cell surfaces and the Raji cells showed the highest level of expression. The flow cytometry analysis using the antiserum to human IFN α/β receptor more clearly showed a strong IFN α/β receptor expression on Raji cells and a weak expression on H9 and U937 cells. These results indicate that B cells (Raji) expressed more receptor molecules on their cell surface than on T cells (H9) and on monocytes (U937), and the number of receptor molecules expressed on the cells is enough to be measured with flow cytometry.
