摘要
Intracellular water volume is an important parameter for expressing concentration of intracellular ions and diffusional water permeability of cells by nuclear magnetic resonance(NMR). Presented here, is a method using 35 Cl and deuterium ( 2D) NMR spectra of cell free supernatant and cell suspension to get intracellular water volume. The volume of intracellular water as a fraction of the total cell volume, V i /V c , in isosmotic solution is 0 706±0 065.
Intracellular water volume is an important parameter for expressing concentration of intracellular ions and diffusional water permeability of cells by nuclear magnetic resonance(NMR). Presented here, is a method using 35 Cl and deuterium ( 2D) NMR spectra of cell free supernatant and cell suspension to get intracellular water volume. The volume of intracellular water as a fraction of the total cell volume, V i /V c , in isosmotic solution is 0 706±0 065.
