细胞因子诱导的杀伤细胞对慢性髓性白血病细胞的体外净化作用

Purging Impact on Chronie Myelogenous Leukemic Cells Mediated by Cytokine Induced Killer Cells

在长期骨髓培养(LTBMC)7—9天的基础上,配对比较观察细胞因子诱导的杀伤细胞(CIK)+白细胞介素2(IL-2)或淋巴因子激活的杀伤细胞(LAK)+IL-2或IL-2活化的骨髓细胞或单纯LTBMC对慢性髓性白血病(CML)患者骨髓Ph^+细胞的净化作用。结果为LTBMC+CIK+IL-2组(n=8)细胞的Ph^+百分率明显低于单纯LTBMC组(n=8,P<0.001),也低于LTBMC+LAN+IL-2组(n=3)或单纯IL-2(n=3)。结论:CIK+IL-2与LTBMC对CML患者骨髓Ph^+细胞的净化有明显的协同作用。

The purging impact of cytokine induced killer (CIK) cells and long term bone, marrow cul.ture (LTBMC) on chronic myelogenous leukemia (CML) patients' Ph + cells was. investigated. First, the bone marrow mononuclear cells of CML patients were cuhured in the following different conditions : ①LTBMC only, ② LTBMC + CIK + interleukin.2 (IL-2), ③ LTBMC +: lymphokine activated killer (LAK) celis + IL - 2, ④ LTBMC + IL - 2; then the chrombsome karyotype was prepared and ana-lyzed for the percentage of Ph+ cells. The percentage of Ph+. cells treated with LTBMC + CIK + IL - 2 ( n = 8) is significantly lower than that with LTBMC only (n= 8, P< 0.001 ) ; it also is lower than, that with LTBMC + LAK + IL - 2 (n = 3 ) or LTBMC + IL - 2 (n = 3 .). The data indicate that CIK + IL - 2 could be used for purgihg of CML cells ex vivo or in adoptive immunotherapy of CML in vivo.