宫颈癌细胞中人乳头瘤病毒感染对DNA含量及细胞周期的影响

EFFECT OF HPV ON HOST CELL DNA PLOIDY AND CELL PHASE FRACTIONS IN CERVICAL CARCINOMA

目的探讨人乳头瘤病毒（ＨＰＶ）感染在宫颈癌变过程中的作用。方法应用聚合酶链反应（ＰＣＲ）和流式细胞计量术（ＦＣＭ）对３９例宫颈癌标本中ＨＰＶ１６和１８型感染、ＤＮＡ含量和细胞周期分布进行检测和分析。结果３１例鳞癌中ＨＰＶ１６阳性１１例（３５．５％），ＨＰＶ１８阳性１例；７例腺癌中ＨＰＶ１６全部阴性，ＨＰＶ１８阳性２例（２８．６％）；１例腺鳞癌ＨＰＶ１６和１８均阴性。总的异倍体率为５９％，二倍体率为４１％。鳞癌异倍体率为７１％（２０／３１），腺癌为４３％（３／７）。鳞癌中ＨＰＶ１６阳性组与阴性组间ＤＮＡ指数和Ｓ期比率有明显差异，而异倍体率无差异。结论ＨＰＶ感染与宫颈癌发生有关。

To study the role of human papilloma virus (HPV)infection in the malignant change ofthe uterine cervix.METHODS Using PCR and FCM techniques,DNA contents and cycle phase were analysed in 39cases of cervical carcinomas infected by HPV 16 and 18.RESULTS The positive rate of HPV 16 in the squamous cell carcinomas was 35. 5 % (11/31 ),that ofHPV 18 in adenocarcinomas was 28. 6% (2/7 ). In 31 cases of squamous cell carcinomas, significantdifferences were found in the mean DNA index and S-phase fraction between the HPV 16 positive groupand negative group, whereas there was no difference in the aneuploidy rate.CONCLUSIONS There exists a relation between HPV infection and cervical cancer change.