水果中三唑酮残留量的气相色谱和气相色谱——质谱分析

Analysis of Triadimefon Residues in Fruits by GC and GC/MS

水果中三唑酮残留用丙酮提取,经液—液分配净化,用气相色谱—电子捕获检测器(GC—ECD)测定,并用气相色谱—质谱(离子选择监测)[Gc/MS(SIM))进行确证。以HP-5大口径毛细管柱和HP-5MS毛细管柱为分离柱,选择出合适的色谱条件,三唑酮的分离效果良好。本方法对苹果样品的测定低限为0.05mg/kg,添加浓度为0.05～1.0mg/kg的三唑酮,回收率为96％以上,变异系数小于4.0％。

The triadimefon residues in fruits was extracted by acetone. After clean-up by liquid-liquid partition, the triadimefon was determined by GC-ECD, and identified by GC/MS (SIM). The detection limit was 0. 05mg/kg for the apple samples. When the fortifying concentration of triadimefon was in the range of 0. 05—1.0mg/kg, the recovery was>96%, and coefficient of variation was<4%.The sample extraction and clean-up were performed according to the microchemical method (MiCCM). The m/z 293, 208, 181 were selected as MS (SIM) monitor ions.