摘要
This paper describes a versatile sensing membrane for immobilizationof proteins (antibodies, antigens, enzymes and so on) and a new immobolizationmethod' The membrane was developed with polyacrylaminde gel and blotinstrep-tavidin system. Streptavidin was bond to the polyacryamide gel with 1-ethyl-3-(3-dimethylaminopropyl carbodimide (EDAC), which results in a streptavidin membrane, and a biotinalyted ligand was then easily linked to the gel via the streptavidin molecules.The streptavidin membrane (the thickness or 500urn) was used toimmobilize anti-human chorionic gonadotrophin antibody (Q hCG). The number orQbCG in the membrane was about 1011 per mm2 of the membrane surface. The membrane was used as a sensing membrane or optical immunosensor (Mach-Zehnder interferomter). It had a good response at the concentration or 10 afl/L hCG.
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作者
Y.K. Zhou, J. W. Yuan,S. Q. Xu, B. H. Shu(Department of Environmental Medicine,Tongji Medical University,Wuhan 430030, China)P. R. H. Kooyman(Department of Applied Physics,MESA Institute, University of Twente, P. O. Box 217,7500 AE Enschede, The Netherlan
