聚合酶链反应检测HBV-DNA及临床意义的再认识

Detection of HBV-DNA by Polymerase Chain Reaction and Cinicd Significance

采用聚合酶链反应检测114例肝病患者血清HBV-DNA。结果:血清HBV-DNA阳性率为35.96％,其中AH,CPH、CAH、LC组HBV-DNA阳性率分别为41.67％、21.43％、53.66％和26.31％;HBeAg阳性组为39.02％;抗HBe阳性组为22.73％;HBsAg、抗HBc阳性组为47.37％;抗HBs阳性组为41.18％;HBVM阴性组为11.11％。结果提示:①CAH组阳性率较高(53.66％);③抗HBe阳性患者血清中有较高滴度HBV-DNA,仍有传染性;③抗HBe阳性、抗HBs阳性、HBVM阴性的患者HBV-DNA阳性可能为HBV的变异株感染。

The serum HBV-DNA were detected by polymerase Chain reaction in 114 cases of liver diseases.The results were as follows ;The positive rates of serum HBV-DNA in 114 cases were 35.96%.The positive incidence in AH,CPH,CAH and LC were 41.67%,21.43%,53.66% and 26.31 % respectively.The positive rates of HBeAg(+),Anti-HBe(+),HBsAg(4)and Anti-HBc(+),Anti-HBs(+),HBVM(-)groups were 39.02%,22.73%,47-37%,41.18% and 11.11% respectively.These results reveal that;(1)the positive rate of CAH was high(53.66%).(2)there were high levels HBV-DNA in serum of Anti-HBe(+)group and the patients are in infectivity yet.(3)the positives of serum HBV-DNA in Anti-HBe(+).Anti-HBs(+)and HBVM(-)groups may be infectived by variant HBV.