日本血吸虫重组抗原保护性免疫力的诱导

INDUCTION OF PROTECTIVE IMMUNITY OF SCHISTOSOMA JAPONICUM RECOMBINANT ANTIGENS IN MICE

采用日本血吸虫重组抗原加福氏佐剂免疫小鼠,攻击感染后,不仅可减少虫负荷(减虫率为29.2％—51.0％),还可降低血吸虫雌虫的产卵量(减卵率为52.1％—74.3％),抗体滴度在初次免疫后3wk即显著升高,并持续维持较高水平。结果表明,经大肠杆菌表达的日本血吸虫重组抗原能够诱导抗攻击感染的保护性免疫力,可望作为混合多价疫苗的候选组分。

Schistosoma japonicum recombinant antigens were used to immunize mice in order to ob-serve their protective immunity against challenge. It was assumed that the recombinant anti-gens could reduce worm burden (29. 2%- 51. 0%) and inibit the fecundity of female schisto-some (52. 1% - 74. 3%). The antibody titers increased significant 3 weeks after the first im-munization and remained high lever. The results indicated that the recombinant antigens ex-pressed in the E. coli could induce protective immunity against challenge. It might be pro-duced in large scale as an important candidate component of a multivalent vaccine against schistosomiasis japonicum.