晾晒烟接种赤星病后游离氨基酸动态的研究

The Study on Free Amino Acid Dynamics of Alternaria Alternata Inoculated on Sun-cured Tobacco

烟株接菌后各类及各种游离氨基酸含量均明显升高,但各有其规律,芳香与杂环族两类增值在侵入期,酸性、碱性、脂肪族及侧键带正电荷四类增值在侵入、潜育到发病初期,侧健为非极性与侧链不带电荷两类自侵入至发病盛期整个病程都增值,各种氨基酸增值的规律大体同各自所属类别,部分有出入,增值峰期在侵入期与发病初期,增值量也随病程增进而递降,到发病盛期时绝大多数氨基酸的含量都低于对照。苯、酪、赖、脯、胱等与抗病性关系较密切的氨基酸增值出现期早、量高、消减也快,仅增值在侵入期或侵入与潜育两个病程,蛋、组、天、丝、甘、丙等氨基酸的增值可持续到发病盛期。

The free amino acids contents increased apparently in the leaves of tobacco after Alternaria alternata inoculated. The contents of aromatic and heterocyclic amino acids were increased in the periods of pathogeny intrusion. The contents of acidic, basic, aliphatic and the lateral chain having positive charge amino acids were increased in the periods of the pathogeny intrusion, incubation and the beginning of the disease come on. The contents of non-polar lateral chain and un-charged lateral chain amino acids were increased throughout the course of disease. The increased valus were got lower with the course of disease, at the vigorous period of disease the most amino acid contents were lower than that of the control. The contents of Phc, Tyr, Lys, Pro, Cys and so on were increased more and earlier and decreased quickly. They only increased at period of intrusion or the periods of intrusion and incubation. The increased contents of Met, His, Asp, Ser, Gly, Ala and so on sustained to at the vigorous periods of disease.