特异性i-RNA预防烧伤感染的作用及其机理的研究

Study about the effect and mechanism of specific i-RNA on preventing infection of burn

为探讨特异性免疫核糖核酸(i-RNA)预防烧伤患者感染的作用及机理,将51例烧伤患者随机分成2组。对照组采用常规治疗,治疗组在常规治疗基础上应用特异性i-RNA。并采用McAb ApAAp法、MTT比色法等技术,观察了2组烧伤患者伤后感染的发生率及免疫功能的变化。结果:(1)治疗组创面细菌培养阳性率及菌血症的发生率显著低于对照组;(2)治疗组全身情况及创面愈合时间也明显优于对照组;(3)治疗组于伤后第10天所测各项免疫指标基本恢复正常水平,对照组各项免疫指标仍明显异常。以上结果2组比较,差异非常显著(P<0.01)。提示特异性i-RNA对烧伤感染有一定的预防作用,可从细胞分子水平纠正烧伤患者的免疫功能紊乱,文中对其机理进行了讨论。

In order to explore the effect and mechanism of specific immune RNA (i-RNA) on preventing infection of burn, 51 burn cases were divided at random into two groups, the control group treated with routine method and the therapeutic group given specific i-RNA on the basis of routine method. Both groups were studied for the infection rate and immune function by the method of monoclonal antibody (McAb) A-pAAp and MTT colorimetery. The results showed : (1) the positive rate(23. 1 % ) of the bacterial culture and the incidence rate (7. 7%) of bacteriemia postburn in the therapeutic group were remarkably lower than those in the control group; (2) the general condition was better and the healing time was shorter in the therapeutic group than those in the control group; (3) by the 10th day postburn,the various immune indexes had approximately restored to normal in the therapeutic group while those in the control group were still at a low level. The difference between the two groups was statistically significant (f <0. 01). It is demonstrated that specific i-RNA might play an important role in preventing infection of burn and reverse immunosuppression caused by burn at the cell molecular level . The mechanism of specific i-RNA augmenting immune function is also discussed.