高效液相色谱法同时测定食品中5种激素

Method for determination of sex hormones in food by HPLC /

建立了食品中雌三醇、雌二醇、雌酮、睾酮、孕酮５种激素同时分离测定的高效液相色谱分析方法。各类食品样品经固一液、液一液萃取处理后，以甲醇一乙腈—四氢呋喃—０．０１ｍｏｌ／Ｌ醋酸钠溶液为流动相，安眠酮为内标进行反相液相色谱分析。５种激素分离效果良好，各组分浓度１．０～３２μｇ／ｍＬ标准曲线线性关系好，回归分析的相关系数均大于０．９９９。方法的最低检测浓度：雌三醇０．００９ｍｇ／ｋｇ；雌二醇０．０３８ｍｇ／ｋｇ；雌酮０．０４４ｍｇ／ｋｇ；睾酮０．０２０ｍｇ／ｋｇ；孕酮０．０７１ｍｇ／ｋｇ。５种激素同时分离测定的相对标准偏差小于１５％不同浓度５种激素同时回收的回收率均在８０％以上。该方法操作简便、准确、灵敏，满足实际样品测定需要。应用该方法对市场上的部分食品分析的结果表明，一些营养保健食品中会有不同成分的激素，提示有关部门应尽快制定法规进行监督管理。

method for determination of sex hormones in food stuff was reported.Samples was extracted with solid-liquid and liquid一liquid extraction, useing hyminalas internal standard substance and RP-HPLC separation was performed on C18 ascolumn with methanol-methyl cyanide-tetrahydrofuran-.01 mol/L sodium acetate asmobile phase,5 compositions (estriol,estradilo,estrone, prgesterone,testosterone) canbe successfully separated. Between 1.0μ g/mL and 3μg/mL,the linear relationshipof standard curve was satisfied for each composition (Relativc Coeffieient > 0.999).Thedetection limit respectively were: estriol 0.009mg/kg;estradiol 0.038mg/kg; estrone0.044mg/kg; testosterone 0. 02mg/kg; progesterone 0.071 mg/kg.The recovery rate forevery composition in different content level was more than 80%,The coeffieient ofvariation obtained for every composition was less than l5%。 This method is simple,accurate and suitable for routine analysis。Author's address Tu Xiaoming, Hygiene and Anti-epidemic Station of Bcijing,Beijing 100013.P.R.C.