用单克隆抗体验证椰毒假单胞菌酵米面亚种O抗原型的初步研究

Preliminary studies on O antigens of Pseudomonas cocovenenans subsp. farinofermentans verified by McAb/

用椰毒假单胞菌种特异性Ｏ－Ⅰ因子及型特异性Ｏ－Ⅳ因子的单克隆抗体Ｐｃｏ６５和Ｐｃｏ１３对从中毒银耳、正常玉米面及动物饲料中新分离的９３株椰毒假单胞菌的菌体抗原型进行了验证。ＥＬＩＳＡ判定结果表明，除１株经再次生化验证鉴定为非椰毒假单胞菌呈阴性反应外，９２株菌均含有种特异性Ｏ－Ⅰ因子，阳性符合率为１００％，其中，４７株菌含有Ｏ－Ⅳ型特异因子，占总菌数的５１．０９％，比血清学分型率（４０．２３％）高出１０．８７％。研究结果表明，鉴定椰毒假单胞菌Ｏ抗原型时、联合应用种间和型间特异性单克隆抗体进行ＥＬＩＳＡ检测，可同时定种分型，方法灵敏、快速，重复性良好。

he somatic antigens of 93 new straims of Pseudomonas cocovenenans subsp.farinofementans isolated from poisoning tremella, corn flour and feed were verified by monoclonal antibodis Pco 65 and Pco 131 Which against O-1 species antigen factor and O-O-Ⅳ type antigen factor seperately. ELISA test results showed that 92 strains had O-1factor except one strain which was not belong to the species of Pseudomonas cocovene-nans in later tests. So the conformabl rate was 100 precent. 47 out of them had O -Ⅳ factor. The serotyping rate(40.2 percent ) by McAb was 10.87 percent highr than by factor serium,It was indicated that species and type of Pseudomonas cocovenenans strains could be test and verify simultaneously when both of O-monoclonal antibodies were used together, and it was sensitive, rapid and good repeated.Author's address Liu Xiumei, Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050,PRC