摘要
A senitive and accurate quantitative method of alphafetoprotein(AFP), enzymo-rocket electrophoretic assay (EREA), was developed by introducing horseradish peroxidase-labeled anti-human AFP antibody into rocket electrophoresis.The lower limit of quantitation ty this method is about 10 ng/ml of AFP.The dose-response curve covers a broader range of concentrations of AFP (10-4000ng/ml)than RIA(0-400 ng/ml) .The accuracy and precision of comparable to that of RIA(r=0.986).Serum AFP measured in 100 patients with primary liver cancer by this method,88% had levels over 25ng/ml.The crossed affinity enzymoimmunoelectrphoresis is a combination of lentil lectin(LCA)affinity electrophoresis and enzymo-rocket electrophoresis,it has been possible to separate the AFP into two variants,LCA-reactive(LCA-R)and LCA-nonreactive (LCA-N)fractions.The advantages of this method are high sensitivity,rapid(6-7h),and can be effectively used to differentiate the primary liver cancer and benign liver disease.
A senitive and accurate quantitative method of alphafetoprotein(AFP), enzymo-rocket electrophoretic assay (EREA), was developed by introducing horseradish peroxidase-labeled anti-human AFP antibody into rocket electrophoresis.The lower limit of quantitation ty this method is about 10 ng/ml of AFP.The dose-response curve covers a broader range of concentrations of AFP (10-4000ng/ml)than RIA(0-400 ng/ml) .The accuracy and precision of comparable to that of RIA(r=0.986).Serum AFP measured in 100 patients with primary liver cancer by this method,88% had levels over 25ng/ml.The crossed affinity enzymoimmunoelectrphoresis is a combination of lentil lectin(LCA)affinity electrophoresis and enzymo-rocket electrophoresis,it has been possible to separate the AFP into two variants,LCA-reactive(LCA-R)and LCA-nonreactive (LCA-N)fractions.The advantages of this method are high sensitivity,rapid(6-7h),and can be effectively used to differentiate the primary liver cancer and benign liver disease.
