摘要
The Xp.2 region of the human X chromosome contains genes involved in a number ofinherited diseases and at least one locus that escapes X chromosome inactivation as well asabnormal methylation polymorphism.We isolated a series of yeast artificial chromosome(YAC)clones by hybridization screening with DNA probes localized within this region andassemblied them into a 4.3 Mb contig spanning from Xp11.21 to Xp11.23 by a combinationof Alu-PCR fingerprinting and DNA probe cross hybridization.On the basis of these over-lapping YAC clones,we have constructed the long-range restriction map of this interval andexactly placed some DNA markers.Four CpG-dense regions between ARAF1 and OATL2were identified by the long-range restriction mapping,which indicated the distribution ofgenes within this interval.It should assist in the future nucleic acid sequence analysis andnovel gene identification in this region.
The Xp.2 region of the human X chromosome contains genes involved in a number of inherited diseases and at least one locus that escapes X chromosome inactivation as well as abnormal methylation polymorphism.We isolated a series of yeast artificial chromosome (YAC)clones by hybridization screening with DNA probes localized within this region and assemblied them into a 4.3 Mb contig spanning from Xp11.21 to Xp11.23 by a combination of Alu-PCR fingerprinting and DNA probe cross hybridization.On the basis of these over- lapping YAC clones,we have constructed the long-range restriction map of this interval and exactly placed some DNA markers.Four CpG-dense regions between ARAF1 and OATL2 were identified by the long-range restriction mapping,which indicated the distribution of genes within this interval.It should assist in the future nucleic acid sequence analysis and novel gene identification in this region.
基金
the high Technology Research and Development Programme of china
National Natural Science Foundation of china
