PEG Delivery of a Wheat Storage Protein Gene into Rice Protoplasts

An efficient cell suspension culture system has been initiated using callus obtained from mature seeds of the Chinese Japonica variety Eryi 105 as a source. Over the last 12 months, using the ″Nottingham Method″, protoplasts have been produced from these cell suspensions, and via the PEG method of transformation. 45 regenerated plants were obtained from DNA-treated protoplasts selected by 80μg/ml G418. The research of molecular analysis on those plantlets is being carried on by Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, U.K.

An effiecent cell suspension culture system has been initiated using callus obtained frommature seeds of the Chinese Japonica variety Eryi 105 as a source.Over the last 12 months,using the'Nottingham Method',protoplasts have been produced from these cell suspen-sions,and via the PEG method of transformation.45 regenerated plants were obtained fromDNA-treated protoplasts selected by 80μg/ml G418.The research of molecular analysis onthose plantlets is being carried on by Plant Genetic Manipulation Group,Department of LifeScience,University of Nottingham,U.K.