胎脑黑质脑内移植矫正PD鼠纹状体内脑啡肽mRNA过度表达

THE CORRECTION OF THE OVER EXPRESSION OF ENKEPHALIN mRNA IN THE STRIATUMOF PD RAT BRAIN FOLLOWING THE IMPLANTATION OF FETAL SUBSTANTIA NIGRA

用６－ＯＨＤＡ损毁大鼠一侧黑质—纹状体通路可引起ＰＤ模型鼠脑纹状体内多巴胺匿乏．同时，亦可导致脑啡肽ｍＲＮＡ过度表达。我们用地高辛标记的ｃＲＮＡ探针对纹状体内脑啡肽ｍＲＮＡ含量进行了斑点杂交定量研究，发现损伤侧脑啡肽ｍＲＮＡ含量较健侧增高８０％，胎中脑移植到失神经支配的纹状体内，脑啡肽ｍＲＮＡ过度表达得以矫正至正常水平，说明胎多巴胺能神经元脑内移植能够调节脑啡肽基因表达，提供了移植物能与宿主发生神经整合、建立突触联系的间接证据。

Unilateral lesion of the nigrostriatal dopamine(DA)pathway induced by 6-hydroxydopamine (6-OHDA) caused significant over expression of enkephalin messenger RNA (mRNA) levels and the deletion of DA in the denervated striatum in rat brain of Parkinson's disease model. Usning a dot blot hybridization technigue by DIG-Lablled cRNA probe, wequantified the contents of enkephalin in the striatum.We found that the levels of enkephalinmRNA after 10 weeks denervation were about 80% higher in the operated lateral striatumthan that in the contralateral one.The implantation of fetal nigral dopaminergic neurons intothe denervated striatum led to a complete correction over-expression of enkephalin mRNA tonormal levels.It indicated that the implantation of fetal DA neurons could regulate the expression of enkephalin gene, providing an indirect evidence that implants could integrate withthe host and rebuild synaptic connections.