小儿先天性巨结肠症酶组织化学变化的研究

THE STUDY OF ENZYME-HISTOCHEMICAL CHANGE OFHIRSCHSPRUNG'S DISEASE IN CHILDREN

小儿先天性巨结肠症（ＨＤ）之病因不甚清楚。国内外学者对其狭窄段各种神经递质的研究较多。而针对细胞能量代谢有关酶类变化研究很少。故本实验对２０例ＨＤ病儿的狭窄段，正常段全层组织进行酶组织化学方法的观察，根据酶反应的强弱分组对比观察。结果发现：（１）ＡＴＰａｓｅ（腺苷三磷酸酶），ＳＤＨ（琥珀酸脱氢酶），Ｃｈｅ（胆碱脂酶），狭窄段酶活性显著高于正常段。（２）ＭＡＯ（单胺氧化酶）活性狭窄段明显低于正常设。（３）狭窄段粘膜下及肌间神经丛，和神经节细胞平均个数明显少于正常段。

Enzyme-histochemical change of Hirschsprung's Disease(HD)was studied by using histochemical staining techniques,Tissues were obtained from 20 operated patients ill with HD.The results showed that:(1)The activities of the cholinsterase(ChE),adenosinetriphosphase(ATPase)and succinicdehydrogenase(SDH) were higher in the stenosed segment than in the normal segment;(2)Monamine oxidase(MAO)activity was lower in thestenosed segment than in the normal segment;(3)The average number of ganglionic cellswas reduced significantly in the intermuscular plexus and submucusal plexus in the stenosedsegment.This study demonstrates that the ATPase,SDH,MAO activities detected with enzyme-histochemical method is one of the new methods in the diagnosis of HD.