尿液中硝基安定、氯硝基安定和氟硝基安定的SepPak HPLC分析

Analysis of Benzodiazepines in Human Urine by SepPak HPLC

本文介绍了一种用SepPak固相萃取技术和HPLC同时检验尿液中的苯并二氮草类药物硝基安定、氯硝基安定和氟硝基安定的方法。使用的反相色谱柱为YMC A—303C_(18)柱,以乙腈—0.01M醋酸铵(45:55,V/V)为流动相,紫外检测波长为254nm。各药物的回收率均大于80％;日内和日间测定的变异系数为1—5％。

A simultaneous analysis of nitrazepam, clonazepam, and flunitrazepam in human urine by SepPak solid-phase extraction and RP-HPLC is presented.Within-day and day-day precision are about.1-5%.It is sensitive and can be used in clinical and toxic kinetic studies.