天然人白细胞介素-2的诱生与纯化

The Induction and Purification of Nature Human Interleukin-2 (IL-2)

用扁桃腺细胞诱生白细胞介素－2（IL2），较适宜的条件是细胞数5×106／ml，PHA100μg／ml，加白细胞干扰素200IU／ml起动，37℃5％CO2培养36～48小时，pH不低于7．0，能较相对稳定地获得平均活性＞700～1，200IU／ml的IL2，最高效价可达2，200IU／ml。以诱生的粗IL2（2，000IU／ml），用硫酸铵80％盐析及DEAN．Sspharose离子交换层析二步法部分纯化，比活性即可达即达＞3．0×106IU／mg。回收率≈60％。本法简易、快捷，用作提纯IL2是较好的方法。

This study was on the induction and purification of human IL-2 produced by tonsil lymphocytes. We found that the optimal cnditions for IL-2 induction were: Cells from tonsil in solution pH≥7.0 and at the concentration of 5×106/ml, adding PHA 100μg/ml, initiation IFN 200IU/ml, incubating the cells at 37℃ for 36-48 hours under 5%CO2 atmosphere. The average titer of Such induced IL-2 got over 700-1, 200IU/ml and the highest titer reached 2, 200IU/ml. The coarse IL-2 (2, 000IU/ml) was partly purified with (NH4)2SO4 precipitation and DEAE-Sepharose chromatography, the specific activity of purified IL-2 was over 3.0× 105IU/mg, receiving rate 60%. We regarded this method as a simply, rapid and good one for purification of nature IL-2.