四种重组造血生长因子对小鼠巨核细胞集落的影响

EFFECTS OF FOUR RECOMBINANT HEMATOPOIETIC GROWTH FACTORS ON MURINE MEGAKARYOCYTE COLONY-FORMING UNITS (CFU-MK)

小鼠体外巨核系祖细胞培养表明,重组鼠白细胞介素-3(IL-3),粒-巨噬细胞集落刺激因子(GM-CSF)、重组人白细胞介素-6(IL-6)及红细胞生成素(EPO)均有不同程度刺激巨核系祖细胞生长的活性。其最适浓度分别为100U/ml,5ng/ml,20ng/ml与1U/ml。对巨核细胞集落刺激作用最强的是IL-3,IL-6与GM-CSF次之,EPO最弱。在种植2×10~5骨髓单个核细胞中分别获得45±3,25±2,20±3及10±2个巨核细胞集落。

Effects of various hematopoietic growth factors including recombinant mouse interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), and recombinant human interleukin-6 (IL-6) and erythropoietin (EPO) on the growth of murine CFU-MK were studied using the plasma clot culture system. IL-3, GM-CSF, IL-6 and EPO each was able to stimulate the formation of megakaryocyte colonies in cultures. The concentrations inducing an optimal growth of CFU-MK are 100 U/ml for IL-3, 5 ng/ml for GM-CSF, 20 ng/ml for IL-6 and 1 U/ml for EPO, respectively. These results indicate that IL-3, GM-CSF, IL-6 and EPO regulate positively megakaryocytopoiesis in vitro.