GENE THERAPY (continued) Ⅱ. TECHNIQUES FOR GENE THERAPY

An essential prerequisite of any genetherapy technique is the efficient and stable de-livery of transferred DNA into the target cell.Avariety of techniques are available for directly in-troducing DNA into eukaryotic cells,includingcoprecipitation with calcium phosphate,electroporation and microinjection. The efficien-cy of most of these physical transfection tech-niques is less than one in ten thousand, and