多聚链式反应(PCR)技术在骨髓移植中的应用
摘要
多聚酶链式反应(PCR)技术是近年来分子生物学领域中应用极广的一项由Mullis等设计的生物学技术。这项技术自1985年以来不仅在生物学领域,而且在医学领域都得到了广泛的应用,其中包括目前在国内外临床上广泛用于血液系统恶性疾患治疗的骨髓移植。 PCR技术是一种模拟天然DNA复制过程的核酸扩增法。即在体外一定的条件下,在待扩增的目的DNA片段两侧加一对寡核苷酸引物,通过变性使DNA成为单链。
出处
《中国实验血液学杂志》
CAS
CSCD
1994年第1期5-8,共4页
Journal of Experimental Hematology
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