星形细胞瘤瘤细胞形态定量测量的研究

Nuclear Morphometry of Astrocytoma by Micro-Image Analysis System

应用微机图象分析系统（MIAS）对40例星形细胞瘤（Ⅰ－Ⅳ）级进行了自动形态测量。选用瘤细胞核的面积、周长、最大径与最小径，核在X或Y轴上的投影长度、形状因子及等效圆直径等8项形态参数，客观地分析了瘤细胞核特征（核的大小和形状）的变化。本研究表明：（1）以瘤细胞核特征作为星形细胞瘤分级的重要指标，是一个既简单、重复性又好的方法；（2）通过MIAS对核特征的客观评估，既可辅助诊断，又可推测预后：（3）MIAS可作为病理工作者对肿瘤进行客观分级的重要工具。

Morphometric image analysis of nuclear features was performed on tissues from 40 cases of astrocytomas which were subdivided into four grades. Eight morphologic parameters of tumor cell nuclei, such as Area, Perimeter,Dmax &. Dmin, Feret X, Feret Y,Cirsf, and Dcircl, were objectively measured and analysed by Micro -Image Analysis System (MIAS). The morphometric data recorded were statistically checked,depending on the tumor grade. The means of Area,Perimeter,Dcircl, Feret X, Feret Y, Dmx,and Dndn were found to grow with significance along with increasing tumor grade up from I to IV. The relative standard deviation(SD) of them were also high -accuracy parameters for the pathologists to describe variability of size and polymophism of nuclei. These parameters show a significant increase of values in parallel with rising tumorgrade. The collclusions were as follows: (1)The use of nuclear grade as prognostic indicator for astrocytoma could provide Such a simple and reproducible method of tumor grading. (2)May objectively evaluate the nuclear characteristics through MIAS,so it could help to diagnose and to predict the prognosis. (3)MIAS is a very useful tool for pathologists to sett1e the problems of tumor grading,which works fast,reliable and reproducible.