摘要
R-phycocyanin (RPC) of Porphyra haitanensis (T. J. Chang et B. F. Zheng )' was chromatographed on Bio-Rex 70 column with urea solution (pH 3. 0) as an eluent, and a and β two subunits were isolated.Their molecular weights were determined on SDS-PAGE at 18 400 and 20 500, respetively,while those of a and β subunits of allophycocyanin (APC) at 18 800 and 19 700, respectively,and those of RPC and APC were at 117 000 and 122 000,respectively.Both the molar ratio of a and β subests of RPC and APC were 1:1, and the subunit composition was confirmed to be (αβ )3.It was ascertained that in RPC αsubunit contains one chromophore phycocyanobilin (PCB) and β subunit has one chromophore PCB and one phycoerythrobilin (PEB), while in APC both α and βsubunits contain one PCB.
R-phycocyanin (RPC) of Porphyra haitanensis (T. J. Chang et B. F. Zheng )' was chromatographed on Bio-Rex 70 column with urea solution (pH 3. 0) as an eluent, and a and β two subunits were isolated.Their molecular weights were determined on SDS-PAGE at 18 400 and 20 500, respetively,while those of a and β subunits of allophycocyanin (APC) at 18 800 and 19 700, respectively,and those of RPC and APC were at 117 000 and 122 000,respectively.Both the molar ratio of a and β subests of RPC and APC were 1:1, and the subunit composition was confirmed to be (αβ )3.It was ascertained that in RPC αsubunit contains one chromophore phycocyanobilin (PCB) and β subunit has one chromophore PCB and one phycoerythrobilin (PEB), while in APC both α and βsubunits contain one PCB.
