摘要
本文以辣根过氧化物酶（PO）作抗原与相应抗体（抗-PO）混合，加入血清，对血清补体抑制免疫复合物沉淀作用（IIPC）进行了研究。结果表明，在抗原抗体最适比例时形成免疫复合物最多；原血清及1∶2稀释血清IIPC最强，OD值为0．57和0．54，血清的进一步稀释对IIPC有一定影响；孵育时间以30min最宜，OD值为0．56；离心时间在25～45min时OD值最大，为0．50～0．56；在最适条件下，正常人混合血清OD值为0．49；56℃30min灭活血清为0．14；50℃15min处理血清为0．38；ED－TA处理血清为0．09，EGTA处理血清为0．21；对20例健康人血清IIPC测定结果为0．39～0．62，平均0．48±0．07。表明正常血清补体对免疫复合物的沉淀有明显抑制作用，各种因素处理血清对IIPC均有一定影响，而以56℃30min和EDTA处理血清最明显。文中还对该法的影响因素进行了讨论。
In this paper, a method for measuring inhibition of immune precipitation by complement(IIPC) using peroxidase (PO) as a antigen was studies. The results showed that immune complex of PO and anti-PO rabbit IgG at equivalence could form a great quantity of precipitation. OD values were 0. 57and 0. 54 in non and diluted serum(1: 2 ), 0. 56 in 30 minutes incubation time, and 0. 50～0. 56 in 25～45 minutes centrifugation time. OD value of normal individuals mixture seruml the serum of heating to 56℃ for 30 minutes, 50℃ 15 minutes, and treated by ethylenediamine tetracetate (EDTA), ethyleneglycol tetracetate(EGTA) were 0. 49, 0. 14, 0. 38, 0. 09, 0. 21 respectively. IIPC of 20 normal persons was 0. 48 ± 0. 07 (0. 39 ～ 0. 62). These results indicate that normal serum markedly inhibites precipitation of immune complex and many agents may block IIPC of serum, especially 56℃ for 30 minutes and EDTA. The various factors of inhibiting IIPC are discussed in the paper.
出处
《河南科技大学学报（医学版）》
1994年第1期3-7,共5页
Journal of Henan University of Science & Technology:Medical Science
基金
河南省卫生厅资助
