肝脏病患者补体抑制免疫沉淀作用的临床研究

Clinical study on inhibition of immune precipitation by complement of hepatopaths

用辣根过氧化物酶（PO）作抗原，与相应抗体抗－PO混合后，加入血清，通过孵育、离心，取上清波加入邻苯二胺，以光电比色法对116例肝脏病患者补体抑制免疫沉淀作用（IIPC）进行了研究。结果，与正常对照组（OD值0．507±0．069）相比，急性肝炎IIPC有所降低，但无显著差异（P＞0．05）；慢性活动性肝炎、慢性迁延性肝炎、重症肝炎IIPC均显著降低（P＜0．001）；肝硬化IIPC下降亦较明显（P＜0．05）。上述各种肝脏病IIPC低下的发生率分别为32％、52％、71％、72％、28％。表明各种肝脏病IIPC均有所降低，并与其严重程度有关。因此认为IIPC的降低在肝脏病的发生发展中起一定作用。

In this paper, using peroxidase(PO) as an antigen, the PO, anti-PO and serum were mixed. After incubation and centrifugation, O-phenylendiamine was added to suPernate. The OD value Was measured by photoelectric colormter. The inhibition of immune pRecipitation by complement(IIPC) of 116hepatopaths was studied. At the same time, C3 and C4 of serum were determined by simple agar diffution. In comporison with control 8roup(0. 507 ±0. 069), the acute hepatitis(AH) IIPC was slightly lower and there was no marked difference(P> 0. 05) ; all the chronic active hepatitis(CAH), chronic persistent hepatitis(CPH) and severe hepatitis (SH) were markedly decreased (P< 0. 001 ); hepatocirrhotis was significantly reduced (P<0. 05). The rates of lower IIPC in AH, CAH,CPH,SH and hepatocirrhocis were 32 %, 52 % , 71 % , 72 %, 28 % respectively. These results suggest that the IIPC in all hepatopaths is decreased, and is related to the patient' s condition.